Fluorescence in situ hybridization on vibratome sections of plant tissues

  title={Fluorescence in situ hybridization on vibratome sections of plant tissues},
  author={P. Vega Prieto and Graham Moore and Peter Shaw},
  journal={Nature Protocols},
This protocol describes the application of fluorescence in situ hybridization (FISH) to three-dimensionally (3D) preserved tissue sections derived from intact plant structures such as roots or florets. The method is based on the combination of vibratome sectioning with confocal microscopy. The protocol provides an excellent tool to investigate chromosome organization in plant nuclei in all cell types and has been used on tissues of both monocot and dicot plant species. The visualization of 3D… CONTINUE READING
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