Fluorescence in situ hybridization in diluted and flow cytometrically sorted boar spermatozoa using specific DNA direct probes labelled by nick translation.

@article{Parrilla2003FluorescenceIS,
  title={Fluorescence in situ hybridization in diluted and flow cytometrically sorted boar spermatozoa using specific DNA direct probes labelled by nick translation.},
  author={Inmaculada Parrilla and Juan Mar{\'i}a V{\'a}zquez and Mar{\'i}a Oliver-Bonet and J. C. Pontes Navarro and Jos{\'e} Y{\'e}lamos and Jordi Roca and Enrique Alcal{\'a} Mart{\'i}nez},
  journal={Reproduction},
  year={2003},
  volume={126 3},
  pages={
          317-25
        }
}
Successful evaluation of X- and Y-chromosome-bearing sperm separation technology using flow cytometry-cell sorter is of great importance. Fluorescence in situ hybridization (FISH), which allows for the detection of specific nucleic acid sequences on morphologically preserved spermatozoa, is an ideal method for quantitatively and qualitatively assessing the purity of sorted sperm samples. In this study specific pig DNA direct probes for small regions of chromosomes 1 and Y were used. Chromosome… CONTINUE READING

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situ hybridization Molecular Reproduction and Development

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