Fluorescence in situ hybridization (FISH) for direct visualization of bacteria in periapical lesions of asymptomatic root-filled teeth.

@article{Sunde2003FluorescenceIS,
  title={Fluorescence in situ hybridization (FISH) for direct visualization of bacteria in periapical lesions of asymptomatic root-filled teeth.},
  author={Pia Titterud Sunde and Ingar Olsen and Ulf Berthold G{\"o}bel and Dirk Theegarten and Sascha Winter and Gilberto J. Debelian and Leif Tronstad and Annette Moter},
  journal={Microbiology},
  year={2003},
  volume={149 Pt 5},
  pages={1095-102}
}
Whether micro-organisms can live in periapical endodontic lesions of asymptomatic teeth is under debate. The aim of the present study was to visualize and identify micro-organisms within periapical lesions directly, using fluorescence in situ hybridization (FISH) in combination with epifluorescence and confocal laser scanning microscopy (CLSM). Thirty-nine periapical lesions were surgically removed, fixed, embedded in cold polymerizing resin and sectioned. The probe EUB 338, specific for the… CONTINUE READING