Fluorescence imaging using a fluorescent protein with a large Stokes shift.

@article{Kogure2008FluorescenceIU,
  title={Fluorescence imaging using a fluorescent protein with a large Stokes shift.},
  author={Takako Kogure and Hiroyuki Kawano and Yukiko S Abe and Atsushi Miyawaki},
  journal={Methods},
  year={2008},
  volume={45 3},
  pages={223-6}
}
Keima is a far-red fluorescent protein endowed with a large Stokes shift. It absorbs light maximally at around 440nm and emits maximally at around 620nm. While the original Keima is obligately tetrameric (tKeima), the dimeric and monomeric versions (mKeima and dKeima, respectively) have been generated. More recently, a tandem dimer of Keima (tdKeima) has been developed as the brightest version. Here we describe examples, which show the usefulness of Keima for dual-color fluorescence imaging… CONTINUE READING

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