Fluorescence fluctuations of quantum-dot sensors capture intracellular protein interaction dynamics

Abstract

We extend the in vitro principle of co-immunoprecipitation to quantify dynamic protein interactions in living cells. Using a multiresolution implementation of fluorescence correlation spectroscopy to achieve maximal temporal resolution, we monitored the interactions of endogenous bait proteins, recruited by quantum dots, with fluorescently tagged prey. With… (More)
DOI: 10.1038/nmeth.1441

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Cite this paper

@article{Zamir2010FluorescenceFO, title={Fluorescence fluctuations of quantum-dot sensors capture intracellular protein interaction dynamics}, author={Eli Zamir and Piet H M Lommerse and Ali Kinkhabwala and Hern{\'a}n E. Grecco and Philippe I. H. Bastiaens}, journal={Nature Methods}, year={2010}, volume={7}, pages={295-298} }