Fluorescence fluctuation spectroscopy.

@article{Chen1999FluorescenceFS,
  title={Fluorescence fluctuation spectroscopy.},
  author={Y Chen and J. S. Bauer S. Virmani D. K. Mueller and Keith M. Berland and Enrico Gratton},
  journal={Methods},
  year={1999},
  volume={19 2},
  pages={234-52}
}
The analysis of the intensity fluctuation of a fluorescence signal from a relatively small volume and from a few molecules contains information about the distribution of different species present in the solution and about kinetic parameters of the system. The same information is generally averaged out when the fluorescence experiment is performed in a much larger volume, typically a cuvette experiment. The fundamental reason for this difference is that the fluctuations of the fluorescence… CONTINUE READING