Fluorescence fluctuation analysis of Arabidopsis thaliana somatic embryogenesis receptor-like kinase and brassinosteroid insensitive 1 receptor oligomerization.

@article{Hink2008FluorescenceFA,
  title={Fluorescence fluctuation analysis of Arabidopsis thaliana somatic embryogenesis receptor-like kinase and brassinosteroid insensitive 1 receptor oligomerization.},
  author={Mark A. Hink and Khalid Shah and Eugenia Russinova and Sacco C de Vries and Antonie J. W. G. Visser},
  journal={Biophysical journal},
  year={2008},
  volume={94 3},
  pages={1052-62}
}
Receptor kinases play a key role in the cellular perception of signals. To verify models for receptor activation through dimerization, an experimental system is required to determine the precise oligomerization status of proteins within living cells. Here we show that photon counting histogram analysis and dual-color fluorescence cross correlation spectroscopy are able to monitor fluorescently labeled proteins at the single-molecule detection level in living plant cells. In-frame fusion… CONTINUE READING

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