Fluorescence energy transfer reveals microdomain formation at physiological temperatures in lipid mixtures modeling the outer leaflet of the plasma membrane.

@article{Silvius2003FluorescenceET,
  title={Fluorescence energy transfer reveals microdomain formation at physiological temperatures in lipid mixtures modeling the outer leaflet of the plasma membrane.},
  author={John R Silvius},
  journal={Biophysical journal},
  year={2003},
  volume={85 2},
  pages={1034-45}
}
An approach is described using fluorescence resonance energy transfer (FRET) to detect inhomogeneity in lipid organization, on distance scales of the order of tens of nanometers or greater, in lipid bilayers. This approach compares the efficiency of energy transfer between two matched fluorescent lipid donors, differing in their affinities for ordered versus disordered regions of the bilayer, and an acceptor lipid that distributes preferentially into disordered regions. Inhomogeneities in… CONTINUE READING

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