Fluorescence energy transfer immunoassay based on a long-lifetime luminescent metal-ligand complex.

@article{Youn1995FluorescenceET,
  title={Fluorescence energy transfer immunoassay based on a long-lifetime luminescent metal-ligand complex.},
  author={Hyun Joo Youn and Ewald A. Terpetschnig and Henryk Szmacinski and Joseph R. Lakowicz},
  journal={Analytical biochemistry},
  year={1995},
  volume={232 1},
  pages={24-30}
}
We describe an immunoassay based on fluorescence resonance energy transfer (FRET). The antigen was human serum albumin (HSA), which was labeled with a ruthenium-ligand complex, [Ru(bpy)2(phen-ITC)]2+. The antibody (IgG) to HSA was labeled with a nonfluorescent absorber, Reactive Blue 4. Association of the Ru-labeled HSA with the antibody was detected by three spectral parameters, a decreased quantum yield of Ru-HSA, a decrease in its fluorescence lifetime, and an increase in its fluorescence… CONTINUE READING