Fluorescence energy transfer between subfragment-1 and actin points in the rigor complex of actosubfragment-1.

@article{Takashi1979FluorescenceET,
  title={Fluorescence energy transfer between subfragment-1 and actin points in the rigor complex of actosubfragment-1.},
  author={Rafael Takashi},
  journal={Biochemistry},
  year={1979},
  volume={18 23},
  pages={5164-9}
}
The fast-reacting thiol (SH1) of myosin subfragment-1 (S-1) was covalently and specifically labeled with (iodoacetamido)fluorescein (IAF), while Cys-373 of actin was also covalently and preferentially labeled with N-(iodoacetyl)-N'-(1-sulfo-5-naphthyl)ethylenediamine (1,5-IAEDANS). The method of fluorescence energy transfer was used to examine the spatial proximity between the two sites, i.e., SH1 and Cys-373, in the rigor complex of acto-S-1. Approximately 30% fluorescence energy transfer was… CONTINUE READING

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