Fluorescence emission properties of S-Layer enhanced green fluorescent fusion protein as a function of temperature, pH conditions, and guanidine hydrochloride concentration.

@article{TocaHerrera2006FluorescenceEP,
  title={Fluorescence emission properties of S-Layer enhanced green fluorescent fusion protein as a function of temperature, pH conditions, and guanidine hydrochloride concentration.},
  author={Jos{\'e} L. Toca-Herrera and Seta K{\"u}pc{\"u} and Valerie Diederichs and Gerald E Moncayo and Dietmar Pum and Uwe Sleytr},
  journal={Biomacromolecules},
  year={2006},
  volume={7 12},
  pages={3298-301}
}
The fluorescent properties of the S-layer enhanced green fluorescent fusion protein (rSbpA31-1068/EGFP) were investigated as a function of temperature, pH conditions, and guanidine hydrochloride concentration. These results were compared to the fluorescent properties of the recombinant enhanced green fluorescent protein (EGFP) and an equimolar mixture of the S-layer protein rSbpA and EGFP. The intensity of the fluorescence emission of the EGFP at 510 nm, after excitation at 490 nm, is not… CONTINUE READING