Fluorescence detection with high time resolution: from optical microscopy to simultaneous force and fluorescence spectroscopy.

@article{Gaiduk2007FluorescenceDW,
  title={Fluorescence detection with high time resolution: from optical microscopy to simultaneous force and fluorescence spectroscopy.},
  author={Alexander Gaiduk and Ralf K{\"u}hnemuth and Suren Felekyan and Matthew Antonik and Wolfgang Becker and Volodymyr Kudryavtsev and Carl Sandhagen and Claus A M Seidel},
  journal={Microscopy research and technique},
  year={2007},
  volume={70 5},
  pages={433-41}
}
Picosecond time-resolution fluorescence signal detection over many hours is possible using the time-correlated single photon counting (TCSPC) technique. Advanced TCSPC with clock oscillator set by the pulsed laser and data analysis provides a tool to investigate processes in single molecules on time scale from picoseconds to seconds. Optical imaging techniques combined with TCSPC allow one to study the spatial distribution of fluorescence properties in solution and on a surface. Mechanical… CONTINUE READING