Fluorescence detection with high time resolution: from optical microscopy to simultaneous force and fluorescence spectroscopy.

Abstract

Picosecond time-resolution fluorescence signal detection over many hours is possible using the time-correlated single photon counting (TCSPC) technique. Advanced TCSPC with clock oscillator set by the pulsed laser and data analysis provides a tool to investigate processes in single molecules on time scale from picoseconds to seconds. Optical imaging… (More)

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Cite this paper

@article{Gaiduk2007FluorescenceDW, title={Fluorescence detection with high time resolution: from optical microscopy to simultaneous force and fluorescence spectroscopy.}, author={Alexander Gaiduk and Ralf K{\"u}hnemuth and Suren Felekyan and Matthew Antonik and Wolfgang Becker and V. A. Kudryavtsev and Carl Sandhagen and Claus A. M. Seidel}, journal={Microscopy research and technique}, year={2007}, volume={70 5}, pages={433-41} }