Fluorescence detection techniques for protein kinase assay.

  title={Fluorescence detection techniques for protein kinase assay.},
  author={Yongjin Li and Weihong Xie and Guijie Fang},
  journal={Analytical and bioanalytical chemistry},
  volume={390 8},
Traditional methods for protein kinase (PK) assay are mainly based on use of (32)P-labeled adenosine triphosphate (ATP); applications of such methods are, however, hampered by radioactive waste and short half-life of (32)P-labeled ATP. Therefore non-radioactive methods, such as fluorescence detection techniques are good alternative. In this review, we describe the principles of four fluorescence techniques (fluorescence intensity endpoint measurement, fluorescence resonance energy transfer… CONTINUE READING

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