Fluorescence detection in automated DNA sequence analysis

  title={Fluorescence detection in automated DNA sequence analysis},
  author={Lloyd M. Smith and Jane Z. Sanders and Robert J. Kaiser and P. Hughes and Chris Dodd and Charles R. Connell and C. Heiner and S. Kent and Leroy E. Hood},
  • Lloyd M. Smith, Jane Z. Sanders, +6 authors Leroy E. Hood
  • Published 1986
  • Chemistry, Medicine
  • Nature
  • We have developed a method for the partial automation of DNA sequence analysis. Fluorescence detection of the DNA fragments is accomplished by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymatic DNA sequence analysis. A different coloured fluorophore is used for each of the reactions specific for the bases A, C, G and T. The reaction mixtures are combined and co-electrophoresed down a single polyacrylamide gel tube, the separated fluorescent bands of DNA… CONTINUE READING
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