Fluorescence decay data analysis correcting for detector pulse pile-up at very high count rates

  title={Fluorescence decay data analysis correcting for detector pulse pile-up at very high count rates},
  author={Matthias Patting and Paja Reisch and Marcus Sackrow and Rhys Dowler and Marcelle Koenig and Michael Wahl},
  journal={Optical Engineering},
Abstract. Using time-correlated single photon counting for the purpose of fluorescence lifetime measurements is usually limited in speed due to pile-up. With modern instrumentation, this limitation can be lifted significantly, but some artifacts due to frequent merging of closely spaced detector pulses (detector pulse pile-up) remain an issue to be addressed. We propose a data analysis method correcting for this type of artifact and the resulting systematic errors. It physically models the… 

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The authors thank Sandra Orthaus-Müller (formerly PicoQuant GmbH) for pushing and inspiring the project. This work was partially funded by BMBF grant 13N12672 "tCAm4Life

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