Fluorescence cryo-microscopy: current challenges and prospects

  title={Fluorescence cryo-microscopy: current challenges and prospects},
  author={R. Kaufmann and Christoph Garth Ariane Middel Hans Hagen and Kay Gr{\"u}newald},
  booktitle={Current opinion in chemical biology},
Studying biological structures with fine details does not only require a microscope with high resolution, but also a sample preparation process that preserves the structures in a near-native state. Live-cell imaging is restricted mostly to the field of light microscopy. For studies requiring much higher resolution, fast freezing techniques (vitrification) are successfully used to immobilize the sample in a near-native state for imaging with electron and X-ray cryo-microscopy. Fluorescence cryo… CONTINUE READING
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