Fluorescence correlation spectroscopy. II. An experimental realization

  title={Fluorescence correlation spectroscopy. II. An experimental realization},
  author={Douglas Magde and Elliot L. Elson and Watt W. Webb},
This paper describes the first experimental application of fluorescence correlation spectroscopy, a new method for determining chemical kinetic constants and diffusion coefficients. These quantities are measured by observing the time behaviour of the tiny concentration fluctuations which occur spontaneously in the reaction system even when it is in equilibrium. The equilibrium of the system is not disturbed during the experiment. The diffusion coefficients and chemical rate constants which… 
Fluorescence correlation spectroscopy. I. Conceptual basis and theory
We describe a method for determining chemical kinetic constants and diffusion coefficients by measuring the rates of decay of spontaneous concentration fluctuations. The equilibrium of the system is
Brief introduction to fluorescence correlation spectroscopy.
  • E. Elson
  • Physics, Chemistry
    Methods in enzymology
  • 2013
Fluorescence Correlation Spectroscopy: Principles and Developments
Twenty years ago, fluorescence measurements at low concentrations were difficult due to the weak fluorescence signal and intrinsic fluctuations of the sample. With the development of FCS and its
3-D Fluorescence Correlation Spectroscopy In Bulk Solution
  • H. Qian, E. Elson
  • Physics
    Photonics West - Lasers and Applications in Science and Engineering
  • 1988
Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Photobleaching Recovery (FPR) are closely related technically but have an important difference in principle. The former extracts
Image cross-correlation spectroscopy: A new experimental biophysical approach to measurement of slow diffusion of fluorescent molecules
The technique of image cross-correlation spectroscopy has been applied to measurements of diffusion of fluorescent beads in polymer solution. The technique is based on measurement and analysis of
Fluorescence Correlation Spectroscopy
TIR/FCS is shown to be a feasible technique for measuring absorption/desorption kinetic rates at equilibrium and should prove useful for measuring specific solute-surface kinetic rates.
Fluorescence correlation spectroscopy in living cells
A step-by-step guide to the application of FCS to cellular systems, including methods for minimizing artifacts, optimizing measurement conditions and obtaining parameter values in the face of diverse and complex conditions of the living cell.


Fluorescence correlation spectroscopy. I. Conceptual basis and theory
We describe a method for determining chemical kinetic constants and diffusion coefficients by measuring the rates of decay of spontaneous concentration fluctuations. The equilibrium of the system is
Experimental Study of Reaction Kinetics by Light Scattering. I. The Polarized Rayleigh Component
Spectral studies of scattered laser light have been conducted in multicomponent solutions where spontaneous fluctuations of the various molecular species occur about the chemical equilibrium. The
Fluctuation spectroscopy: determination of chemical reaction kinetics from the frequency spectrum of fluctuations.
  • G. Feher, M. Weissman
  • Chemistry, Physics
    Proceedings of the National Academy of Sciences of the United States of America
  • 1973
Results obtained by this method for the dissociation reaction of beryllium sulfate agree well with those obtained by relaxation methods in which the approach to equilibrium is analyzed.
Light Scattering as a Probe of Fast‐Reaction Kinetics: The Depolarized Spectrum of Rayleigh Scattered Light from a Chemically Reacting Medium
The spectral distribution of the depolarized component of light scattered from a dilute solution of molecules in dynamic chemical equilibrium between two states differing in optical anisotropy and
Thermodynamic Fluctuations in a Reacting System-Measurement by Fluorescence Correlation Spectroscopy
The temporal correlations of thermodynamic concentration fluctuations have been measured in a chemically reactive system at equilibrium by observing fluctuations of the fluorescence of a reaction
Light Scattering from a Chemically Reactive Fluid. I. Spectral Distribution
The spectral distribution of light scattered by fluctuations about equilibrium in a chemically reactive fluid is calculated from the time dependence of the fluctuations predicted by the linearized
Light Scattering from Chemically Reactive Fluids. IV. Intensity Calculations for the Eulerian Fluid with One Reaction
The hydrodynamic equations for the reactive Eulerian fluid (zero transport coefficients, one chemical reaction) have been used in the thermodynamic approach to calculate in detail the spectrum of
Light Scattering from Reactive Fluids. III. Intensity Calculation
Using standard perturbation theory for degenerate matrices, an expansion for the eigenvectors corresponding to the thermodynamic fluctuation modes in a chemically reactive fluid is obtained. The
Light Scattering from Chemically Reacting Fluids: Coupled Chemical Reactions
The total light scattering intensity from a fluid where chemical relaxation takes place via a sequence of coupled reactions is calculated and examined. The fast reaction limiting case where the rate