Fluorescence correlation spectroscopy. I. Conceptual basis and theory

@article{Elson1974FluorescenceCS,
  title={Fluorescence correlation spectroscopy. I. Conceptual basis and theory},
  author={Elliot L. Elson and Douglas Magde},
  journal={Biopolymers},
  year={1974},
  volume={13}
}
We describe a method for determining chemical kinetic constants and diffusion coefficients by measuring the rates of decay of spontaneous concentration fluctuations. The equilibrium of the system is not disturbed during the measurement. We measure the number of molecules of a specified type in a defined open volume as a function of time and compute the time course of the deviations from the thermodynamic mean concentration. The method is based on the principle that the rates of decay of… 
Fluorescence correlation spectroscopy. II. An experimental realization
This paper describes the first experimental application of fluorescence correlation spectroscopy, a new method for determining chemical kinetic constants and diffusion coefficients. These quantities
Brief introduction to fluorescence correlation spectroscopy.
  • E. Elson
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  • 2006
TLDR
In an application to a protein folding reaction, the time-development of the diffusion coefficient was observed during the folding reaction and it was found that the change of the hydrogen bonding network occurred by the two state manner in entire refolding process of cytochrome c.
Fluorescence correlation spectroscopy and nonlinear stochastic reaction-diffusion.
TLDR
The results show that current linear FCS theory could be adequate for measurements on biological systems that contain many other sources of uncertainties, and extends Delbrück-Gillespie's theory for stochastic nonlinear reactions with rapid stirring to reaction-diffusion systems provides a mesoscopic model for chemical and biochemical reactions at nanometric and mesoscopic levels.
Fluorescence Correlation Spectroscopy
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TIR/FCS is shown to be a feasible technique for measuring absorption/desorption kinetic rates at equilibrium and should prove useful for measuring specific solute-surface kinetic rates.
Determination of Equilibrium Constant and Relative Brightness in Fluorescence Correlation Spectroscopy by Considering Third-Order Correlations.
TLDR
This article proposes to combine the third-order FCS with the conventional second- order FCS to determine K and Q without invoking other resources, and demonstrates the strategy and formalism in a classical example of the hairpin DNA-folding process.
Fluorescence Correlation Spectroscopy and Nonlinear Stochastic Reaction − Di ff usion
The currently existing theory of fluorescence correlation spectroscopy (FCS) is based on the linear fluctuation theory originally developed by Einstein, Onsager, Lax, and others as a phenomenological
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