Fluorescence correlation spectroscopy in small cytosolic compartments depends critically on the diffusion model used.

@article{Gennerich2000FluorescenceCS,
  title={Fluorescence correlation spectroscopy in small cytosolic compartments depends critically on the diffusion model used.},
  author={Arne Gennerich and Detlev Schild},
  journal={Biophysical journal},
  year={2000},
  volume={79 6},
  pages={3294-306}
}
Fluorescence correlation spectroscopy (FCS) is a powerful technique for measuring low concentrations of fluorescent molecules and their diffusion constants. In the standard case, fluorescence fluctuations are measured in an open detection volume defined by the confocal optics. However, if FCS measurements are carried out in cellular processes that confine the detection volume, the standard FCS model leads to erroneous results. In this paper, we derive a modified FCS model that takes into… CONTINUE READING