Fluorescence correlation spectroscopy for the detection and study of single molecules in biology.

@article{Medina2002FluorescenceCS,
  title={Fluorescence correlation spectroscopy for the detection and study of single molecules in biology.},
  author={Miguel {\'A}ngel Medina and Petra Schwille},
  journal={BioEssays : news and reviews in molecular, cellular and developmental biology},
  year={2002},
  volume={24 8},
  pages={
          758-64
        }
}
  • M. Medina, P. Schwille
  • Published 1 August 2002
  • Chemistry, Physics, Biology
  • BioEssays : news and reviews in molecular, cellular and developmental biology
The recent development of single molecule detection techniques has opened new horizons for the study of individual macromolecules under physiological conditions. Conformational subpopulations, internal dynamics and activity of single biomolecules, parameters that have so far been hidden in large ensemble averages, are now being unveiled. Herein, we review a particular attractive solution-based single molecule technique, fluorescence correlation spectroscopy (FCS). This time-averaging… 

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