Fluorescence correlation spectroscopy and its potential for intracellular applications

@article{Schwille2001FluorescenceCS,
  title={Fluorescence correlation spectroscopy and its potential for intracellular applications},
  author={Petra Schwille},
  journal={Cell Biochemistry and Biophysics},
  year={2001},
  volume={34},
  pages={383-408}
}
Fluorescence correlation spectroscopy (FCS) is a time-averaging fluctuation analysis of small molecular ensembles, combining maximum sensitivity with high statistical confidence. Among a multitude of physical parameters that are, in principle, accessible by FCS, it most conveniently allows to determine local concentrations, mobility coefficients, and characteristic rate constants of fast-reversible and slow-irreversible reactions of fluorescently labeled biomolecules at very low (nanomolar… CONTINUE READING

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