Fluorescence correlation spectroscopy and its potential for intracellular applications

  title={Fluorescence correlation spectroscopy and its potential for intracellular applications},
  author={Petra Schwille},
  journal={Cell Biochemistry and Biophysics},
Fluorescence correlation spectroscopy (FCS) is a time-averaging fluctuation analysis of small molecular ensembles, combining maximum sensitivity with high statistical confidence. Among a multitude of physical parameters that are, in principle, accessible by FCS, it most conveniently allows to determine local concentrations, mobility coefficients, and characteristic rate constants of fast-reversible and slow-irreversible reactions of fluorescently labeled biomolecules at very low (nanomolar… CONTINUE READING


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Publications referenced by this paper.
Showing 1-10 of 97 references

Statistical accuracy in fluo- rescence correlation spectroscopy

D. E. Koppel
Phys. Rev. A • 1974
View 4 Excerpts
Highly Influenced

Characterization of photo-induced isomeriza- tion and back-isomerization of the cyanine dye Cy5 by fluorescence correlation spectroscopy

J. Widengren, P. Schwille
J. Phys. Chem • 2000
View 3 Excerpts

Cross-correlation analysis in FCS

P. Schwille
Theory and applications (Elson, • 2000
View 2 Excerpts

EGFP and DsRed expressing cultures of E. coli by confocal, two- photon and fluorescence lifetime microscopy

S. Jakobs, V. Subramanian, A. Schönle, T. M. Jovin, S. W. Hell
FEBS Lett • 2000
View 1 Excerpt

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