Fluorescence correlation spectroscopy: the technique and its applications

@article{Krichevsky2002FluorescenceCS,
  title={Fluorescence correlation spectroscopy: the technique and its applications},
  author={Oleg Krichevsky and Gr{\'e}goire Bonnet},
  journal={Reports on Progress in Physics},
  year={2002},
  volume={65},
  pages={251-297}
}
Fluorescence correlation spectroscopy (FCS) is an experimental technique using statistical analysis of the fluctuations of fluorescence in a system in order to decipher dynamic molecular events, such as diffusion or conformational fluctuations of biomolecules. First introduced by Magde et al to measure the diffusion and binding of ethidium bromide onto double-stranded DNA, the technique has been undergoing a renaissance since 1993 with the implementation of confocal microscopy FCS. Since then… 
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