Fluorescence correlation spectroscopy: past, present, future.

  title={Fluorescence correlation spectroscopy: past, present, future.},
  author={Elliot L. Elson},
  journal={Biophysical journal},
  volume={101 12},
In recent years fluorescence correlation spectroscopy (FCS) has become a routine method for determining diffusion coefficients, chemical rate constants, molecular concentrations, fluorescence brightness, triplet state lifetimes, and other molecular parameters. FCS measures the spatial and temporal correlation of individual molecules with themselves and so provides a bridge between classical ensemble and contemporary single-molecule measurements. It also provides information on concentration and… CONTINUE READING

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