Fluorescence correlation spectroscopy: detecting and interpreting the mobility of transmembrane proteins in vivo.

@article{Malchus2011FluorescenceCS,
  title={Fluorescence correlation spectroscopy: detecting and interpreting the mobility of transmembrane proteins in vivo.},
  author={Nina Malchus},
  journal={Current protocols in toxicology},
  year={2011},
  volume={Chapter 2},
  pages={Unit2.19}
}
Mobility of proteins is crucial for their functionality. Fluorescence correlation spectroscopy (FCS) is a sensitive tool for assessing dynamics in vivo. It can reveal properties of diffusing proteins, as well as of the surrounding medium. Hence, subtle changes in the dynamics after treatment with toxic substances can be visualized. On biological membranes, the high concentration of transmembrane and peripheral membrane proteins leads to molecular crowding, and thus to a change in the diffusion… CONTINUE READING

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