Fluorescence correlation spectrometry of the interaction kinetics of tetramethylrhodamin alpha-bungarotoxin with Torpedo californica acetylcholine receptor.

@article{Rauer1996FluorescenceCS,
  title={Fluorescence correlation spectrometry of the interaction kinetics of tetramethylrhodamin alpha-bungarotoxin with Torpedo californica acetylcholine receptor.},
  author={Barbara Rauer and Eberhard Neumann and Jerker Widengren and Rudolf W. Rigler},
  journal={Biophysical chemistry},
  year={1996},
  volume={58 1-2},
  pages={
          3-12
        }
}
Fluorescence correlation spectroscopy (FCS) is suited to determine low concentrations (10(-8) M) of slowly interacting molecules with different translational diffusion coefficients on the level of single molecule counting. This new technique was applied to characterize the interaction dynamics of tetramethylrhodamin labelled alpha-bungarotoxin (B( *)) with the detergent solubilized nicotinic acetylcholine receptor (AChR) of Torpedo californica electric organ. At pseudo-first-order conditions… CONTINUE READING
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