Fluorescence-based microtiter plate assay for glutamate-cysteine ligase activity.

@article{White2003FluorescencebasedMP,
  title={Fluorescence-based microtiter plate assay for glutamate-cysteine ligase activity.},
  author={Collin C. White and Hannah Viernes and Cecile M. Krejsa and D. Botta and Terrance J Kavanagh},
  journal={Analytical biochemistry},
  year={2003},
  volume={318 2},
  pages={175-80}
}
Glutamate-cysteine ligase (GCL; also known as gamma-glutamylcysteine synthetase) is the rate-limiting enzyme in glutathione (GSH) synthesis. Traditional assays for the activity of this enzyme are based either on coupled reactions with other enzymes or on high-performance liquid chromatography (HPLC) assessment of gamma-glutamylcysteine (gamma-GC) product formation. We took advantage of the reaction of naphthalene dicarboxaldehyde (NDA) with GSH or gamma-GC to form cyclized products that are… CONTINUE READING

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