Fluorescence-based melting assays for studying quadruplex ligands.

  title={Fluorescence-based melting assays for studying quadruplex ligands.},
  author={Anne De Cian and Lionel Guittat and Markus Kaiser and Barbara Sacc{\`a} and Samir Amrane and Anne Bourdoncle and P. Alberti and Marie-Paule Teulade-Fichou and Laurent Lacroix and Jean-Louis Mergny},
  volume={42 2},
The telomeric G-rich single-stranded DNA can adopt in vitro an intramolecular quadruplex structure, which has been shown to directly inhibit telomerase activity. The reactivation of this enzyme in immortalized and most cancer cells suggests that telomeres and telomerase are relevant targets in oncology, and telomere ligands and telomerase inhibitors have been proposed as new potential anticancer agents. In this paper, we have analysed the FRET method used to measure the stabilization and… CONTINUE READING

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