Fluorescence-based assays of lipases, phospholipases, and other lipolytic enzymes.


In choosing an assay, one needs to consider the following questions: What level of sensitivity is required? Must the assay be continuous? Is the substrate readily available; can it be purchased or must it be custom synthesized? How specific is the substrate? How convenient is the method? How compatible is it with monomolecular, micellar, or vesicular… (More)