Fluorescence-based assay for the interaction of small molecules with the human renal organic anion transporter 1.

@article{Cihl2000FluorescencebasedAF,
  title={Fluorescence-based assay for the interaction of small molecules with the human renal organic anion transporter 1.},
  author={Tom{\'a}{\vs} Cihl{\'a}ř and Edmund S Ho},
  journal={Analytical biochemistry},
  year={2000},
  volume={283 1},
  pages={49-55}
}
Secretion of small molecules from the systemic blood circulation into urine is one of the physiologically essential functions of the kidney. The human organic anion transporter (hOAT1) is a key component in the renal tubular secretion of negatively charged molecules including a variety of important therapeutics. In some cases, compounds interacting with hOAT1 may induce pharmacokinetic drug-drug interactions or cause nephrotoxicity. We developed a fluorescence-based, 96-well format assay using… CONTINUE READING