Fluorescence aptasensor based on competitive-binding for human neutrophil elastase detection.

@article{He2010FluorescenceAB,
  title={Fluorescence aptasensor based on competitive-binding for human neutrophil elastase detection.},
  author={Jing-lin He and Zaisheng Wu and Song-Bai Zhang and Guo-Li Shen and Ruqin Yu},
  journal={Talanta},
  year={2010},
  volume={80 3},
  pages={1264-8}
}
To our knowledge, we report the first fluorescence aptasensor for detecting human neutrophil elastase (HNE) in homogeneous solution. The biosensor contains a short DNA scrambled sequence strand (SS) complementary to part of the aptamer sequence or the loop of molecular beacon (MB). The aptamer-HNE recognition event involves competition between the molecular… CONTINUE READING