Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity.

  title={Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity.},
  author={J. F. Baret and Oliver J. Miller and Val{\'e}rie Taly and Micha{\"e}l Ryckelynck and Abdeslam El-Harrak and Lucas Frenz and Christian Rick and Michael L. Samuels and J. Brian Hutchison and Jeremy J. Agresti and Darren R. Link and David A Weitz and Andrew D. Griffiths},
  journal={Lab on a chip},
  volume={9 13},
We describe a highly efficient microfluidic fluorescence-activated droplet sorter (FADS) combining many of the advantages of microtitre-plate screening and traditional fluorescence-activated cell sorting (FACS). Single cells are compartmentalized in emulsion droplets, which can be sorted using dielectrophoresis in a fluorescence-activated manner (as in FACS) at rates up to 2000 droplets s(-1). To validate the system, mixtures of E. coli cells, expressing either the reporter enzyme beta… CONTINUE READING


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