Fluorescence activated cell sorting of plant protoplasts.

  title={Fluorescence activated cell sorting of plant protoplasts.},
  author={Bastiaan O. R. Bargmann and Kenneth D. Birnbaum},
  journal={Journal of visualized experiments : JoVE},
High-resolution, cell type-specific analysis of gene expression greatly enhances understanding of developmental regulation and responses to environmental stimuli in any multicellular organism. In situ hybridization and reporter gene visualization can to a limited extent be used to this end but for high resolution quantitative RT-PCR or high-throughput transcriptome-wide analysis the isolation of RNA from particular cell types is requisite. Cellular dissociation of tissue expressing a… CONTINUE READING


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