Fluorescence-activated cell sorting for aptamer SELEX with cell mixtures

@article{Mayer2010FluorescenceactivatedCS,
  title={Fluorescence-activated cell sorting for aptamer SELEX with cell mixtures},
  author={G{\"u}nter Mayer and Marie-Sophie L Ahmed and Andreas Dolf and Elmar Endl and Percy A Knolle and Michael Famulok},
  journal={Nature Protocols},
  year={2010},
  volume={5},
  pages={1993-2004}
}
Aptamers that target a specific cell subpopulation within composite mixtures represent invaluable tools in biomedical research and in the development of cell-specific therapeutics. Here we describe a detailed protocol for a modular and generally applicable scheme to select aptamers that target the subpopulations of cells in which you are interested. A fluorescence-activated cell-sorting device is used to simultaneously differentiate and separate those subpopulations of cells having bound and… CONTINUE READING
46 Citations
59 References
Similar Papers

References

Publications referenced by this paper.
Showing 1-10 of 59 references

Similar Papers

Loading similar papers…