Fluorescence-activated cell sorting for aptamer SELEX with cell mixtures

  title={Fluorescence-activated cell sorting for aptamer SELEX with cell mixtures},
  author={G{\"u}nter Mayer and Marie-Sophie L Ahmed and Andreas Dolf and Elmar Endl and Percy A Knolle and Michael Famulok},
  journal={Nature Protocols},
Aptamers that target a specific cell subpopulation within composite mixtures represent invaluable tools in biomedical research and in the development of cell-specific therapeutics. Here we describe a detailed protocol for a modular and generally applicable scheme to select aptamers that target the subpopulations of cells in which you are interested. A fluorescence-activated cell-sorting device is used to simultaneously differentiate and separate those subpopulations of cells having bound and… CONTINUE READING
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