Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ.

@article{Nolan1988FluorescenceactivatedCA,
  title={Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ.},
  author={Garry P. Nolan and Steven Fiering and Jean François Nicolas and Leonard A. Herzenberg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1988},
  volume={85 8},
  pages={2603-7}
}
We demonstrate that individual cells infected with and expressing a recombinant retrovirus carrying the Escherichia coli beta-galactosidase gene (lacZ) can be viably stained, analyzed, sorted, and cloned by fluorescence-activated cell sorting based on the levels of lacZ expressed. To accomplish this we have devised a method to enzymatically generate and… CONTINUE READING