Fluorescence Resonance Energy Transfer Based on Interaction of PII and PipX Proteins Provides a Robust and Specific Biosensor for 2-Oxoglutarate, a Central Metabolite and a Signaling Molecule.

@article{Chen2013FluorescenceRE,
  title={Fluorescence Resonance Energy Transfer Based on Interaction of PII and PipX Proteins Provides a Robust and Specific Biosensor for 2-Oxoglutarate, a Central Metabolite and a Signaling Molecule.},
  author={Hai-Lin Chen and Christophe S{\'e}bastien Bernard and Pierre Hubert and Laetitia My and Cheng-Cai Zhang},
  journal={The FEBS journal},
  year={2013}
}
2-Oxoglutarate is a central metabolite and a signalling molecule in both prokaryotes and eukaryotes. The cellular levels of 2-oxoglutarate vary rapidly in response to environmental changes, but an easy and reliable approach is lacking for the measurement of 2-oxoglutarate. Here we report a biosensor of 2-oxoglutarate based on the 2-oxoglutarate-dependent dissociation of the PII-PipX protein complex from cyanobacteria. Fusions of PII and PipX to either CFP or YFP could form a complex and their… CONTINUE READING
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