Fluorescence Recovery Under Decaying Photobleaching Irradiation: Concept and Experiment

@article{Glazachev2006FluorescenceRU,
  title={Fluorescence Recovery Under Decaying Photobleaching Irradiation: Concept and Experiment},
  author={Yu. I. Glazachev and Valery V Khramtsov},
  journal={Journal of Fluorescence},
  year={2006},
  volume={16},
  pages={773-781}
}
A novel modification of photobleaching method for measurement of lateral diffusion is developed. In this approach fluorescence recovery kinetics is measured under decaying photobleaching irradiation, termed as fluorescence recovery under decaying photobleaching (FRDP). The time evolution of fluorescence intensity normalized to input irradiation starts from the photobleaching kinetics and transforms into the kinetics of fluorescence recovery at a later stage resulting in appearance of minimum… CONTINUE READING

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