Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids

@article{Villa2017FluorescenceMO,
  title={Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids},
  author={J. Villa and Hong-Anh Tran and Megha A Vipani and Stephanie L. Gianturco and Konark Bhasin and Brent L. Russell and E. Harbron and D. Young},
  journal={Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry},
  year={2017},
  volume={22}
}
  • J. Villa, Hong-Anh Tran, +5 authors D. Young
  • Published 2017
  • Medicine, Chemistry
  • Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
The ability to modulate protein function through minimal perturbations to amino acid structure represents an ideal mechanism to engineer optimized proteins. Due to the novel spectroscopic properties of green fluorescent protein, it has found widespread application as a reporter protein throughout the fields of biology and chemistry. Using site-specific amino acid mutagenesis, we have incorporated various fluorotyrosine residues directly into the fluorophore of the protein, altering the… Expand
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