Fluorescence-Based Phenotypic Selection Allows Forward Genetic Screens in Haploid Human Cells

@inproceedings{Duncan2012FluorescenceBasedPS,
  title={Fluorescence-Based Phenotypic Selection Allows Forward Genetic Screens in Haploid Human Cells},
  author={Lidia M. Duncan and Richard T Timms and Eszter Zavodszky and Florencia Cano and Gordon Dougan and Felix Randow and Paul J Lehner},
  booktitle={PloS one},
  year={2012}
}
The isolation of haploid cell lines has recently allowed the power of forward genetic screens to be applied to mammalian cells. The interest in applying this powerful genetic approach to a mammalian system is only tempered by the limited utility of these screens, if confined to lethal phenotypes. Here we expand the scope of these approaches beyond live/dead screens and show that selection for a cell surface phenotype via fluorescence-activated cell sorting can identify the key molecules in an… CONTINUE READING