Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase.

@article{Kinoshita1997FluorescenceIO,
  title={Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase.},
  author={Yasunori Kinoshita and Koichi Nishigaki and Yuzuru Husimi},
  journal={Nucleic acids research},
  year={1997},
  volume={25 18},
  pages={3747-8}
}
A rapid 5'-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5'-ends of both oligo- and polynucleotides. 

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 14 extracted citations

RNA-RNA Interactions

Methods in Molecular Biology • 2015
View 8 Excerpts
Highly Influenced

Enzymes used in molecular biology: a useful guide

Journal of Cell Communication and Signaling • 2008

Similar Papers

Loading similar papers…