Fluid transport by human nonpigmented ciliary epithelial layers in culture: a homeostatic role for aquaporin-1.

@article{Patil2001FluidTB,
  title={Fluid transport by human nonpigmented ciliary epithelial layers in culture: a homeostatic role for aquaporin-1.},
  author={Rajkumar V. Patil and Zhaosheng Han and Maimaiti T Yiming and Jian Yang and Pavel Iserovich and M. Wax and Jorge Fischbarg},
  journal={American journal of physiology. Cell physiology},
  year={2001},
  volume={281 4},
  pages={C1139-45}
}
We report for the first time that cultured nonpigmented human ciliary epithelial (NPE) cell layers transport fluid. Cells were grown to confluence on permeable membrane inserts, and fluid transport across the resulting cell layers was determined by volume clamp at 37 degrees C. These cell layers translocated fluid from the apical to the basal side at a steady rate of 3.6 microl x h(-1) x cm(-2) (n = 4) for 8 h. This fluid movement was independent of hydrostatic pressure and was completely… CONTINUE READING