Fluctuations in the microsecond time range of the current through single acetylcholine receptor ion channels

@article{Colquhoun1981FluctuationsIT,
  title={Fluctuations in the microsecond time range of the current through single acetylcholine receptor ion channels},
  author={David Colquhoun and Bert J. Sakmann},
  journal={Nature},
  year={1981},
  volume={294},
  pages={464-466}
}
Acetylcholine-like drugs cause ion channels in the skeletal muscle endplate to open briefly1, producing, at random intervals, rectangular pulses of current with constant amplitude but random duration, that can be recorded by the patch clamp method2,3. However, even when the agonist concentration is so low that channel activations are very well separated, we have observed, with high resolution methods4, that openings may be interrupted by shut periods (gaps) so brief that they are very unlikely… 
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Three types of experiments revealed two kinetic processes controlling the opening of endplate channels, one that reflects agonist action and another that reflects local anesthetic-like blocking activity in decamethonium action.
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