Fluctuating enzymes: lessons from single-molecule studies.

  title={Fluctuating enzymes: lessons from single-molecule studies.},
  author={Wei Min and Brian P English and Guobin Luo and Binny J Cherayil and Samuel C. Kou and Xiaoliang Sunney Xie},
  journal={Accounts of chemical research},
  volume={38 12},
Recent single-molecule enzymology measurements with improved statistics have demonstrated that a single enzyme molecule exhibits large temporal fluctuations of the turnover rate constant at a broad range of time scales (from 1 ms to 100 s). The rate constant fluctuations, termed as dynamic disorder, are associated with fluctuations of the protein conformations observed on the same time scales. We discuss the unique information extractable from these experiments and the reconciliation of these… CONTINUE READING