Flt3-ligand, IL-4, GM-CSF, and adherence-mediated isolation of murine lung dendritic cells: assessment of isolation technique on phenotype and function.

@article{Swanson2004Flt3ligandIG,
  title={Flt3-ligand, IL-4, GM-CSF, and adherence-mediated isolation of murine lung dendritic cells: assessment of isolation technique on phenotype and function.},
  author={Kena A. Swanson and Yan Zheng and K. Heidler and Z. D. Zhang and Tonya J Roberts Webb and David Stephen Wilkes},
  journal={Journal of immunology},
  year={2004},
  volume={173 8},
  pages={4875-81}
}
Lung dendritic cells (DCs) are difficult to study due to their limited quantities and the complexities required for isolation. Although many procedures have been used to overcome this challenge, the effects of isolation techniques on lung DCs have not been reported. The current study shows that freshly isolated DCs (CD11c+) have limited ability to induce proliferation in allogeneic T cells, and are immature as indicated by low cell surface expression of costimulatory molecules compared with… CONTINUE READING