Flt1 acts as a negative regulator of tip cell formation and branching morphogenesis in the zebrafish embryo.

@article{Krueger2011Flt1AA,
  title={Flt1 acts as a negative regulator of tip cell formation and branching morphogenesis in the zebrafish embryo.},
  author={Janna Krueger and Dong Liu and Katja Scholz and Anja Zimmer and Yu Shi and Christian Klein and Arndt F Siekmann and Stefan Schulte-Merker and Melissa Jane Cudmore and Asif Ahmed and Ferdinand le Noble},
  journal={Development},
  year={2011},
  volume={138 10},
  pages={2111-20}
}
Endothelial tip cells guide angiogenic sprouts by exploring the local environment for guidance cues such as vascular endothelial growth factor (VegfA). Here we present Flt1 (Vegf receptor 1) loss- and gain-of-function data in zebrafish showing that Flt1 regulates tip cell formation and arterial branching morphogenesis. Zebrafish embryos expressed soluble Flt1 (sFlt1) and membrane-bound Flt1 (mFlt1). In Tg(flt1(BAC):yfp) × Tg(kdrl:ras-cherry)(s916) embryos, flt1:yfp was expressed in tip, stalk… CONTINUE READING