Flow profile near a wall measured by double-focus fluorescence cross-correlation.

  title={Flow profile near a wall measured by double-focus fluorescence cross-correlation.},
  author={Dirk Lumma and Andreas Best and Alex Gansen and François Feuillebois and Joachim O. R{\"a}dler and Olga I Vinogradova},
  journal={Physical review. E, Statistical, nonlinear, and soft matter physics},
  volume={67 5 Pt 2},
We present an experimental approach to flow profiling within femtoliter sample volumes, which allows the high-precision measurements at the solid interface. The method is based on the spatial cross-correlation of the fluorescence response from labeled tracer particles (latex nanospheres or single dye molecules). Two excitation volumes, separated by a few micrometers, are created by two laser foci under a confocal microscope. The velocity of tracer particles is measured in a channel about 100… CONTINUE READING
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