Flow-flash, time-resolved resonance Raman spectroscopy of the oxidation of reduced and of mixed valence cytochrome oxidase by dioxygen.

Abstract

Time-resolved resonance Raman spectroscopy has been used to study the reduction of oxygen by both reduced and mixed valence cytochrome oxidase. Laser flash photodissociation of CO from the carbon monoxy complex of the enzyme, after this species had been rapidly mixed with oxygenated buffer, was used to initiate the reaction for both forms of the enzyme. The… (More)

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