Flow cytometry thresholds of myeloperoxidase detection to discriminate between acute lymphoblastic or myeloblastic leukaemia.

@article{Guy2013FlowCT,
  title={Flow cytometry thresholds of myeloperoxidase detection to discriminate between acute lymphoblastic or myeloblastic leukaemia.},
  author={Julien Guy and Il{\'e}ana Antony-Debr{\'e} and Emmanuel Benayoun and Isabelle Arnoux and Chantal Fossat and Magali Le Garff-Tavernier and Anna Raimbault and Mich{\`e}le Imbert and Marck Maynadi{\'e} and Francis Lacombe and Marie Christine B{\'e}n{\'e} and Orianne Wagner-Ballon},
  journal={British journal of haematology},
  year={2013},
  volume={161 4},
  pages={551-5}
}
The World Health Organization 2008 Classification emphasizes myeloperoxidase (MPO) detection as sufficient for assigning a blast population to the myeloid lineage. Published MPO positivity thresholds are 10% for flow cytometry (FCM) but 3% for cytochemistry. Here we re-evaluated the FCM-MPO threshold by comparing retrospectively 128 acute lymphoblastic leukaemias and 75 acute myeloid leukaemias without maturation, all assessed by benzidine-based cytochemistry. A 13% threshold was found to be… CONTINUE READING