Flow cytometry measurements of human chromosome kinetochore labeling.

@article{Fantes1989FlowCM,
  title={Flow cytometry measurements of human chromosome kinetochore labeling.},
  author={Judy A. Fantes and D. K. Green and Patricia Malloy and Adrian T. Sumner},
  journal={Cytometry},
  year={1989},
  volume={10 2},
  pages={134-42}
}
A method for the preparation and measurement of immunofluorescent human chromosome centromeres in suspension is described using CREST antibodies, which bind to the centromeric region of chromosomes. Fluorescein isothiocyanate (FITC)-conjugated antihuman antibodies provide the fluorescent label. Labeled chromosomes are examined on microscope slides and by… CONTINUE READING