Flow cytometry for real-time measurement of guanine nucleotide binding and exchange by Ras-like GTPases.

@article{Schwartz2008FlowCF,
  title={Flow cytometry for real-time measurement of guanine nucleotide binding and exchange by Ras-like GTPases.},
  author={Samantha L. Schwartz and Mathewos Tessema and Tione Buranda and Olena Pylypenko and Alexey V Rak and Peter M. Simons and Zurab Surviladze and Larry A. Sklar and Angela Wandinger-Ness},
  journal={Analytical biochemistry},
  year={2008},
  volume={381 2},
  pages={258-66}
}
Ras-like small GTPases cycle between GTP-bound active and GDP-bound inactive conformational states to regulate diverse cellular processes. Despite their importance, detailed kinetic or comparative studies of family members are rarely undertaken due to the lack of real-time assays measuring nucleotide binding or exchange. Here we report a bead-based flow cytometric assay that quantitatively measures the nucleotide binding properties of glutathione-S-transferase (GST) chimeras for prototypical… CONTINUE READING

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