Flow cytometry distinction between adherent and phagocytized yeast particles.

@article{Giaimis1994FlowCD,
  title={Flow cytometry distinction between adherent and phagocytized yeast particles.},
  author={Jean Giaimis and Yves Lombard and Philippe Poindron and Christian D Muller},
  journal={Cytometry},
  year={1994},
  volume={17 2},
  pages={173-8}
}
Our laboratory recently developed a light microscopy staining technique that provides a mean to distinguish between yeast that are simply bound to the surface of macrophages and yeast that have actually been phagocytized by macrophages (7). We adapted this technique by using fluorescent probes in order to test phagocytic activity by flow cytometry. Thus we are able to distinguish unambiguously extracellular from intracellular yeast during phagocytosis with the fast rate of flow cytometry… CONTINUE READING